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Characterization of the Arabidopsis thaliana exocyst complex gene families by phylogenetic, expression profiling, and subcellular localization studies
Author(s) -
Chong Yolanda T.,
Gidda Satinder K.,
Sanford Chris,
Parkinson John,
Mullen Robert T.,
Goring Daphne R.
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2009.03070.x
Subject(s) - arabidopsis thaliana , biology , exocyst , phylogenetic tree , arabidopsis , gene , gene expression , gene expression profiling , genetics , subcellular localization , evolutionary biology , computational biology , microbiology and biotechnology , mutant , protein subunit
Summary• The exocyst is a complex of eight proteins (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p and Exo84p) involved in tethering vesicles to the plasma membrane during regulated or polarized secretion. Here, the plant exocyst complex was explored in phylogenetic, expression, and subcellular localization studies. • Evolutionary relationships of predicted exocyst subunits were examined in the complete genomes of Arabidopsis thaliana , Oryza sativa , Populus trichocarpa and Physcomitrella patens . Furthermore, detailed expression profiling of the A. thaliana microarray databases was performed and subcellular localization patterns were studied. • Several plant exocyst subunit genes appear to have undergone gene expansion in a common ancestor and subsequent duplication events in independent plant lineages. Expression profiling revealed that the A. thaliana Exo70 gene family exhibits dynamic expression patterns, while the remaining exocyst subunit genes displayed more static profiles. Subcellular localization patterns for A. thaliana exocyst subunits ranged from cytosolic to endosomal compartments (with enrichment in the early endosomes and the trans‐Golgi network). Interestingly, two endosomal‐localized AtExo70 proteins also recruited other exocyst subunits to these compartments. • Overall subcellular localization patterns were observed that were also found in yeast and animal cells, and this, coupled with the evolutionary relationships, suggests that the exocyst may perform similar conserved functions in plants.