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SPL28 encodes a clathrin‐associated adaptor protein complex 1, medium subunit μ1 (AP1M1) and is responsible for spotted leaf and early senescence in rice ( Oryza sativa )
Author(s) -
Qiao Yongli,
Jiang Wenzhu,
Lee JooHyun,
Park BongSoo,
Choi MinSeon,
Piao Rihua,
Woo MiOk,
Roh JaeHwan,
Han Longzhi,
Paek NamChon,
Seo Hak Soo,
Koh HeeJong
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2009.03047.x
Subject(s) - biology , signal transducing adaptor protein , microbiology and biotechnology , oryza sativa , clathrin , mutant , protein subunit , endomembrane system , callose , golgi apparatus , biochemistry , signal transduction , endocytosis , gene , endoplasmic reticulum , cell
Summary•  To expand our understanding of cell death in plant defense responses, we isolated a novel rice ( Oryza sativa ) spotted leaf mutant ( spl28 ) that displays a lesion mimic phenotype in the absence of pathogen attack through treatment of Hwacheongbyeo (an elite Korean japonica cultivar) with N‐methyl‐N‐nitrosourea (MNU). •  Early stage development of the spl28 mutant was normal. However, after flowering, spl28 mutants exhibited a significant decrease in chlorophyll content, soluble protein content, and photosystem II efficiency, and high concentrations of reactive oxygen species (ROS), phytoalexin, callose, and autofluorescent phenolic compounds that localized in or around the lesions. The spl28 mutant also exhibited significantly enhanced resistance to rice blast and bacterial blight. •  Using a map‐based cloning approach, we determined that SPL28 encodes a clathrin‐associated adaptor protein complex 1, medium subunit μ1 (AP1M1), which is involved in the post‐Golgi trafficking pathway. A green fluorescent protein (GFP) fusion protein of SPL28 (SPL28::GFP) localized to the Golgi apparatus, and expression of SPL28 complemented the membrane trafficking defect of apm1‐1Δ yeast mutants. SPL28 was ubiquitously expressed and contained a highly conserved adaptor complex medium subunit (ACMS) family domain. •  SPL28 appears to be involved in the regulation of vesicular trafficking, and SPL28 dysfunction causes the formation of hypersensitive response (HR)‐like lesions, leading to the initiation of leaf senescence.

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