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A new stable isotope approach identifies the fate of ozone in plant–soil systems
Author(s) -
Toet Sylvia,
Subke JensArne,
D’Haese David,
Ashmore Mike R.,
Emberson Lisa D.,
Crossman Zoe,
Evershed Richard P.,
Barnes Jeremy D.,
Ineson Phil
Publication year - 2009
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2009.02780.x
Subject(s) - apoplast , soil water , ozone , environmental chemistry , environmental science , stable isotope ratio , flux (metallurgy) , chemistry , organic matter , isotope analysis , soil science , ecology , biology , physics , organic chemistry , quantum mechanics , biochemistry , cell wall
Summary• We show that the stable isotope 18 O can be used to trace ozone into different components of the plant–soil system at environmentally relevant concentrations. • We exposed plants and soils to 18 O‐labelled ozone and used isotopic enrichment in plant dry matter, leaf water and leaf apoplast, as well as in soil dry matter and soil water, to identify sites of ozone‐derived 18 O accumulation. • It was shown that isotopic accumulation rates in plants can be used to infer the location of primary ozone‐reaction sites, and that those in bare soils are dependent on water content. However, the isotopic accumulation rates measured in leaf tissue were much lower than the modelled stomatal flux of ozone. • Our new approach has considerable potential to elucidate the fate and reactions of ozone within both plants and soils, at scales ranging from plant communities to cellular defence mechanisms.