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Arabidopsis IRT3 is a zinc‐regulated and plasma membrane localized zinc/iron transporter
Author(s) -
Lin YaFen,
Liang HongMing,
Yang ShuYi,
Boch Annegret,
Clemens Stephan,
Chen ChyiChuann,
Wu JingFen,
Huang JingLing,
Yeh KuoChen
Publication year - 2009
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2009.02766.x
Subject(s) - arabidopsis , arabidopsis thaliana , mutant , zinc , protoplast , transporter , schizosaccharomyces pombe , hyperaccumulator , biology , green fluorescent protein , biochemistry , transport protein , chemistry , microbiology and biotechnology , gene , phytoremediation , organic chemistry , ecology , contamination
Summary• ZIP transporters (ZRT, IRT‐like proteins) are involved in the transport of iron (Fe), zinc (Zn) and other divalent metal cations. The expression of IRT3, a ZIP transporter, is higher in the Zn/cadmium (Cd) hyperaccumulator Arabidopsis halleri than is that of its ortholog in Arabidopsis thaliana , which implies a positive association of its expression with Zn accumulation in A. halleri.• IRT3 genes from both A. halleri and A. thaliana functionally complemented the Zn uptake mutant Spzrt1 in Schizosaccharomyces pombe ; and Zn uptake double mutant zrt1zrt2 , Fe‐uptake mutant fet3fet4 and conferred Zn and Fe uptake activity in Saccharomyces cerevisiae . By contrast, the manganese (Mn) uptake mutant smf1 phenotypes were not rescued. Insufficient Cd uptake for toxicity was found. • Expression of IRT3‐green fluorescent protein (GFP) fusion proteins in Arabidopsis root protoplasts indicated localization of both IRT3 proteins in the plasma membrane. • Overexpressing AtIRT3 in A. thaliana led to increased accumulation of Zn in the shoot and Fe in the root of transgenic lines. Therefore, IRT3 functions as a Zn and Fe‐uptake transporter in Arabidopsis.