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Molecular phylogeny and expression of poplar circadian clock genes, LHY1 and LHY2
Author(s) -
Takata Naoki,
Saito Shigeru,
Tanaka Saito Claire,
Nanjo Tokihiko,
Shinohara Kenji,
Uemura Matsuo
Publication year - 2009
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02714.x
Subject(s) - biology , phylogenetic tree , gene duplication , gene , phylogenetics , evolutionary biology , genetics , plant evolution , synteny , arabidopsis thaliana , molecular clock , mutant , genome
Summary• LHY/CCA1 genes play a key role in the plant circadian clock system and are highly conserved among plant species. However, the evolutionary process of the LHY / CCA1 gene family remains unclear in angiosperms. To obtain details of the phylogeny of these genes, this study characterized LHY/CCA1 genes in a model woody plant, Populus tree. • The evolutionary process of angiosperm LHY/CCA1 genes was elucidated using three approaches: comparison of exon–intron structures, reconstruction of phylogenetic trees and examination of syntenic relationships. In addition, the molecular evolutionary rates and the expression patterns of Populus LHY s were analyzed. • Gene duplication events of Populus LHY s and Arabidopsis LHY/CCA1 had occurred independently by different chromosomal duplication events arising in each evolutionary lineage. Populus LHY s were under purifying selection by estimating substitution rates of these genes. Further, Populus LHY s conserved diurnal expressions in leaves and stems but the transcripts of LHY2 were more abundant than those of LHY1 in Populus plants. • This study uncovered phylogenetic relationships of the LHY / CCA1 gene family in angiosperms. In addition, the transcript abundance and the evolutionary differences between Populus LHY1 and LHY2 imply that Populus LHY2 , rather than LHY1 , may have a major role in the Populus clock system.