Premium
Transcriptome analysis of Medicago truncatula leaf senescence: similarities and differences in metabolic and transcriptional regulations as compared with Arabidopsis , nodule senescence and nitric oxide signalling
Author(s) -
De Michele Roberto,
Formentin Elide,
Todesco Marco,
Toppo Stefano,
Carimi Francesco,
Zottini Michela,
Barizza Elisabetta,
Ferrarini Alberto,
Delledonne Massimo,
Fontana Paolo,
Lo Schiavo Fiorella
Publication year - 2009
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02684.x
Subject(s) - medicago truncatula , senescence , biology , transcriptome , arabidopsis , microbiology and biotechnology , gene , genetics , botany , gene expression , symbiosis , bacteria , mutant
Summary• Here, for the first time, a comprehensive transcriptomics study is presented of leaf senescence in the legume model Medicago truncatula , providing a broad overview of differentially expressed transcripts involved in this process. • The cDNA‐amplification fragment length polymorphism (AFLP) technique was used to identify > 500 genes, which were cloned and sorted into functional categories according to their gene ontology annotation. • Comparison between the datasets of Arabidopsis and M. truncatula leaf senescence reveals common physiological events but differences in the nitrogen metabolism and in transcriptional regulation. In addition, it was observed that a minority of the genes regulated during leaf senescence were equally involved in other processes leading to programmed cell death, such as nodule senescence and nitric oxide signalling. • This study provides a wide transcriptional profile for the comprehension of key events of leaf senescence in M. truncatula and highlights a possible regulative role for MADS box transcription factors in the terminal phases of the process.