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OsIPD3, an ortholog of the Medicago truncatula DMI3 interacting protein IPD3, is required for mycorrhizal symbiosis in rice
Author(s) -
Chen Caiyan,
Ané JeanMichel,
Zhu Hongyan
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02612.x
Subject(s) - medicago truncatula , symbiosis , biology , mutant , oryza sativa , phenotype , lotus japonicus , gene , botany , genetics , bacteria
Summary• Medicago truncatula IPD3 (MtIPD3) is an i nteracting p rotein of D MI 3 ( d oes not m ake i nfections 3), a Ca 2+ /calmodulin‐dependent protein kinase (CCaMK) essential for both arbuscular mycorrhizal (AM) and rhizobial symbioses. However, the function of MtIPD3 in root symbioses has not been demonstrated in M. truncatula , because of a lack of knockout mutants for functional analysis. In this study, the availability of IPD3 knockout mutants in rice ( Oryza sativa ) was exploited to test the function of OsIPD3 in AM symbiosis. • Three independent retrotransposon Tos17 insertion lines of OsIPD3 were selected and the phenotypes characterized upon inoculation with the AM fungus Glomus intraradices . • Phenotypic and genetic analyses revealed that the Osipd3 mutants were unable to establish a symbiotic association with G. intraradices . • In conclusion, IPD3 represents a novel gene required for root symbiosis with AM fungi in plants.