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Barley Rom1 antagonizes Rar1 function in Magnaporthe oryzae ‐infected leaves by enhancing epidermal and diminishing mesophyll defence
Author(s) -
Zellerhoff Nina,
Jansen Marcus,
Schaffrath Ulrich
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02597.x
Subject(s) - blumeria graminis , biology , mutant , powdery mildew , hordeum vulgare , microbiology and biotechnology , plant disease resistance , pathogen , xanthomonas oryzae , gene , genetics , botany , poaceae
Summary• Barley ( Hordeum vulgare ) is a host for Blumeria graminis f. sp. hordei ( Bgh ), which causes powdery mildew, and for the rice blast pathogen Magnaporthe oryzae. It has previously been shown that Rar1 , initially identified in a mutational screen as being r equired for Ml a 12 ‐specified Bgh ‐ r esistance, also controlled pathogenic growth of M. oryzae in barley. Here, we tested whether the rom1 mutation ( r est o ration of M la12 ‐specified resistance), which restored resistance against Bgh in a susceptible rar1‐2 genetic background, also influences the interaction between barley and M. oryzae . • Disease severity after infection with M. oryzae was analysed on rar1‐2 mutants and rar1‐2 rom1 double mutants. Microscopy and northern analysis were used to gain insight into cellular and molecular events. • On rar1‐2 rom1 double mutant plants, the number of M. oryzae disease lesions was increased in comparison to the wild‐type and the rar1‐2 mutant which correlated with augmented epidermal penetration. However, a decrease in the lesion diameter, apparently conditioned in the mesophyll, was also observed. • These results highlight the impact of Rom1 in basal defence of barley against different pathogens. Importantly, a tissue‐specific function for Rom1 with contrasting effects on epidermal and mesophyll defence was demonstrated.