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Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro‐PIXE
Author(s) -
VogelMikuš Katarina,
Regvar Marjana,
MesjaszPrzybyłowicz Jolanta,
Przybyłowicz Wojciech J.,
Simčič Jure,
Pelicon Primož,
Budnar Miloš
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02519.x
Subject(s) - hyperaccumulator , cadmium , epidermis (zoology) , botany , cryofixation , chemistry , cuticle (hair) , zinc , vascular bundle , metal , biomonitoring , trichome , biology , heavy metals , environmental chemistry , phytoremediation , anatomy , organic chemistry , ultrastructure
Summary• Localization of cadmium (Cd) and other elements was studied in the leaves of the field‐collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro‐PIXE (proton‐induced X‐ray emission). • The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole‐leaf cross‐sections and selected tissues were obtained. • Cd was preferentially localized in the lower epidermis (820 µg g −1 DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll. • Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.