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Soybean defense responses to the soybean aphid
Author(s) -
Li Yan,
Zou Jijun,
Li Min,
Bilgin Damla D.,
Vodkin Lila O.,
Hartman Glen L.,
Clough Steven J.
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2008.02443.x
Subject(s) - biology , soybean aphid , pseudomonas syringae , cultivar , gene , aphid , complementary dna , gene expression , plant defense against herbivory , dna microarray , botany , genetics , horticulture , pest analysis , aphididae , homoptera
Summary• Transcript profiles in aphid ( Aphis glycines )‐resistant (cv. Dowling) and ‐susceptible (cv. Williams 82) soybean ( Glycine max ) cultivars using soybean cDNA microarrays were investigated. • Large‐scale soybean cDNA microarrays representing approx. 18 000 genes or c . 30% of the soybean genome were compared at 6 and 12 h post‐application of aphids. In a separate experiment utilizing clip cages, expression of three defense‐related genes were examined at 6, 12, 24, 48, and 72 h in both cultivars by quantitative real‐time PCR. • One hundred and forty genes showed specific responses for resistance; these included genes related to cell wall, defense, DNA/RNA, secondary metabolism, signaling and other processes. When an extended time period of sampling was investigated, earlier and greater induction of three defense‐related genes was observed in the resistant cultivar; however, the induction declined after 24 or 48 h in the resistant cultivar but continued to increase in the susceptible cultivar after 24 h. • Aphid‐challenged resistant plants showed rapid differential gene expression patterns similar to the incompatible response induced by avirulent Pseudomonas syringae . Five genes were identified as differentially expressed between the two genotypes in the absence of aphids.