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Ectopic expression of GmPAP3 alleviates oxidative damage caused by salinity and osmotic stresses
Author(s) -
Li WingYen Francisca,
Shao Guihua,
Lam HonMing
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02356.x
Subject(s) - paraquat , reactive oxygen species , oxidative stress , antioxidant , chemistry , lipid peroxidation , ascorbic acid , biochemistry , mitochondrion , glutathione , osmotic shock , ectopic expression , arabidopsis thaliana , oxidative phosphorylation , microbiology and biotechnology , biology , food science , enzyme , mutant , gene
Summary• The primary biochemical reaction of purple acid phosphatases (PAP) is to catalyze the hydrolysis of phosphate esters and anhydrides. However, the soybean GmPAP3 gene expression is induced by NaCl, osmotic, and oxidative treatments, indicating a possible role of PAP in abiotic stress responses. • Confocal and electron microscopic studies demonstrated that GmPAP3 protein is mainly localized in mitochondria, a primary site for reactive oxygen species (ROS) production. • When subjected to NaCl and polyethylene glycol (PEG) treatments, ectopic expression of GmPAP3 in transgenic tobacco BY‐2 cells mimicked the protective effects exhibited by the antioxidant ascorbic acid: increase in the percentage of cells with active mitochondria; reduction in the percentage of dead cells; and reduced accumulation of ROS. In addition, when GmPAP3 transgenic Arabidopsis thaliana seedlings were subjected to NaCl, PEG, and paraquat (PQ) treatments, the percentage of root elongation was significantly higher than the wild type. Furthermore, PQ‐induced lipid peroxidation in these transgenic seedlings was also reduced. • In summary, the mitochondrial localized GmPAP3 may play a role in stress tolerance by enhancing ROS scavenging.