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Isolate specificity of quantitative trait loci for partial resistance of barley to Puccinia hordei confirmed in mapping populations and near‐isogenic lines
Author(s) -
Marcel Thierry C.,
Gorguet Benoît,
Ta Minh Truong,
Kohutova Zuzana,
Vels Anton,
Niks Rients E.
Publication year - 2008
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02298.x
Subject(s) - biology , quantitative trait locus , hordeum vulgare , cultivar , major gene , botany , population , hordeum , resistance (ecology) , poaceae , gene , rust (programming language) , horticulture , genetics , agronomy , computer science , programming language , demography , sociology
Summary• Partial resistance is considered race‐nonspecific and durable, consistent with the concept of ‘horizontal’ resistance. However, detailed observations of partial resistance to leaf rust ( Puccinia hordei ) in barley ( Hordeum vulgare ) revealed small cultivar × isolate interactions, suggesting a minor‐gene‐for‐minor‐gene interaction model, similar to so‐called ‘vertical’ resistance. • Three consistent quantitative trait loci (QTLs), labelled Rphq2, Rphq3 and Rphq4, that were detected in the cross susceptible L94 × partially resistant Vada have been incorporated into the L94 background to obtain near‐isogenic lines (NILs). Three isolates were used to map QTLs on seedlings of the L94 × Vada population and to evaluate the effect of each QTL on adult plants of the respective NILs under field conditions. • Rphq2 had a strong effect in seedlings but almost no effect in adult plants, while Rphq3 was effective in seedlings and in adult plants against all three isolates. However, Rphq4 was effective in seedlings and in adult plants against two isolates but ineffective in both development stages against the third, demonstrating a clear and reproducible isolate‐specific effect. The resistance governed by the three QTLs was not associated with a hypersensitive reaction. • Those results confirm the minor‐gene‐for‐minor‐gene model suggesting specific interactions between QTLs for partial resistance and P. hordei isolates.