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Nitric oxide induced by hydrogen peroxide mediates abscisic acid‐induced activation of the mitogen‐activated protein kinase cascade involved in antioxidant defense in maize leaves
Author(s) -
Zhang Aying,
Jiang Mingyi,
Zhang Jianhua,
Ding Haidong,
Xu Shucheng,
Hu Xiuli,
Tan Mingpu
Publication year - 2007
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02071.x
Subject(s) - abscisic acid , nitric oxide , mapk/erk pathway , antioxidant , protein kinase a , hydrogen peroxide , sodium nitroprusside , chemistry , microbiology and biotechnology , biochemistry , kinase , nitric oxide synthase , biology , enzyme , organic chemistry , gene
Summary• The role of nitric oxide (NO) and the relationship between NO, hydrogen peroxide (H 2 O 2 ) and mitogen‐activated protein kinase (MAPK) in abscisic acid (ABA)‐induced antioxidant defense in leaves of maize ( Zea mays ) plants were investigated. • Both ABA and H 2 O 2 induced increases in the generation of NO in mesophyll cells of maize leaves, and H 2 O 2 was required for the ABA‐induced generation of NO. Pretreatment with NO scavenger and nitric oxide synthase (NOS) inhibitor substantially reduced the ABA‐induced production of NO, and partly blocked the activation of a 46 kDa MAPK and the expression and the activities of several antioxidant enzymes induced by ABA. Treatment with the NO donor sodium nitroprusside (SNP) also induced the activation of the MAPK, and enhanced the antioxidant defense systems. • Conversely, SNP treatment did not induce the production of H 2 O 2 , and pretreatments with NO scavenger and NOS inhibitor did not affect ABA‐induced H 2 O 2 production. • Our results suggest that ABA‐induced H 2 O 2 production mediates NO generation, which, in turn, activates MAPK and results in the upregulation in the expression and the activities of antioxidant enzymes in ABA signaling.