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Rhodococcus fascians infection accelerates progression of tobacco BY‐2 cells into mitosis through rapid changes in plant gene expression
Author(s) -
Vandeputte Olivier,
Vereecke Danny,
Mol Adeline,
Lenjou Marc,
Van Bockstaele Dirk,
El Jaziri Mondher,
Baucher Marie
Publication year - 2007
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02062.x
Subject(s) - cell cycle , biology , leafy , mitosis , agrobacterium , gene , microbiology and biotechnology , gene expression , plant cell , genetics , botany , transgene
Summary• To characterize plant cell cycle activation following Rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco BY‐2 cells was investigated. • S‐phase‐synchronized BY‐2 cells were cocultivated with R. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. Cell cycle alteration was further investigated by cDNA‐AFLP (amplified fragment length polymorphism). • It was shown that cell cycle progression of BY‐2 cells was accelerated only upon infection with bacteria whose virulence gene expression was induced by a leafy gall extract. Thirty‐eight BY‐2 genes showed a differential expression within 6 h post‐infection. Among these, seven were previously associated with specific plant cell cycle phases (in particular S and G2/M phases). Several genes also showed a differential expression during leafy gall formation. • R. fascians ‐infected BY‐2 cells provide a simple model to identify plant genes related to leafy gall development. R. fascians can also be regarded as a useful biotic agent to alter cell cycle progression and, thereby, gain a better understanding of cell cycle regulation in plants.