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A quantitative trait loci analysis of Zinc hyperaccumulation in Arabidopsis halleri
Author(s) -
Filatov Victor,
Dowdle John,
Smirnoff Nicholas,
FordLloyd Brian,
Newbury H. John,
Macnair Mark R.
Publication year - 2007
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02036.x
Subject(s) - quantitative trait locus , biology , genetics , locus (genetics) , gene , chromosome , arabidopsis , genetic analysis , mutant
Summary• The mechanisms of metal hyperaccumulation are still not understood, so we conducted a quantitative trait locus (QTL) analysis of zinc (Zn) hyperaccumulation in Arabidopsis halleri , in a cross between this and its sister species, A. petraea , in order to determine the number and approximate location of the genomic regions significantly contributing to this adaptation. • An F2 cross between the two species was made, and the leaf Zn concentration of 92 individuals was measured at both low (10 µ m ) and high (100 µ m ) Zn concentrations. Twenty‐five markers were established that were distributed on all of the eight chromosomes. • Mapping of the markers established that they were essentially collinear with previous studies. QTLs exceeding a logarithm to the base 10 of the odds (LOD) value of 3 were found on chromosomes 4 (low Zn), 6 (high Zn) and 7 (both high and low Zn). Evidence for a QTL on chromosome 3 (low Zn) was also found. • This analysis validates a previously used method of QTL analysis, based on microarray analysis of segregating families. Genes that have altered during the evolution of this character should also be QTL: this analysis calls into question a number of candidate genes from consideration as such primary genes because they do not appear to be associated with QTLs.