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The use of the zinc‐fluorophore, Zinpyr‐1, in the study of zinc homeostasis in Arabidopsis roots
Author(s) -
Sinclair Scott A.,
Sherson Sarah M.,
Jarvis Renée,
Camakaris James,
Cobbett Christopher S.
Publication year - 2007
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.02030.x
Subject(s) - endodermis , arabidopsis , pericycle , zinc , xylem , fluorescence , chemistry , fluorophore , arabidopsis thaliana , mutant , homeostasis , biophysics , biology , botany , biochemistry , microbiology and biotechnology , gene , physics , organic chemistry , quantum mechanics
Summary• The usefulness of the zinc (Zn)‐fluorophore, Zinpyr‐1, to examine the localization of Zn in the roots of Arabidopsis has been investigated. • In wild‐type roots Zinpyr‐1 fluorescence was predominantly in the xylem. The fluorescence signal was abolished by the application of the Zn‐chelator, N,N,N’,N‐tetrakis(2‐pyridylmethyl)ethylenediamine (TPEN), and was increased by increasing exogenous Zn in the medium, indicating that fluorescence reflected relative Zn concentrations. • In the hma2 , hma4 double mutant, which is deficient in root to shoot Zn translocation, Zinpyr‐1 fluorescence was low in the xylem and high in the adjacent pericycle cells in which HMA2 and HMA4 are specifically expressed in a wild type. Zinpyr‐1 fluorescence was also increased in the endodermis. • These results show that Zinpyr‐1 can be used to examine the effects of mutations in Zn transporters on the localization of Zn in Arabidopsis roots and should be a useful addition to the tools available for studying Zn homeostasis in plants.