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Early and specific gene expression triggered by rice resistance gene Pi33 in response to infection by ACE1 avirulent blast fungus
Author(s) -
Vergne E.,
Ballini E.,
Marques S.,
Sidi Mammar B.,
Droc G.,
Gaillard S.,
Bourot S.,
DeRose R.,
Tharreau D.,
Nottéghem J.L.,
Lebrun M.H.,
Morel J.B.
Publication year - 2007
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2007.01971.x
Subject(s) - gene , magnaporthe grisea , biology , genetics , gene expression , plant disease resistance , r gene , regulation of gene expression , gene interaction , fungus , oryza sativa , botany
Summary• Our view of genes involved in rice disease resistance is far from complete. Here we used a gene‐for‐gene relationship corresponding to the interaction between atypical avirulence gene ACE1 from Magnaporthe grisea and rice resistance gene Pi33 to better characterize early rice defence responses induced during such interaction. • Rice genes differentially expressed during early stages of Pi33 / ACE1 interaction were identified using DNA chip‐based differential hybridization and QRT‐PCR survey of the expression of known and putative regulators of disease resistance. • One hundred genes were identified as induced or repressed during rice defence response, 80% of which are novel, including resistance gene analogues. Pi33 / ACE1 interaction also triggered the up‐regulation of classical PR defence genes and a massive down‐regulation of chlorophyll a/b binding genes. Most of these differentially expressed genes were induced or repressed earlier in Pi33 / ACE1 interaction than in the gene–for–gene interaction involving Nipponbare resistant cultivar. • Besides demonstrating that an ACE1 / Pi33 interaction induced classical and specific expression patterns, this work provides a list of new genes likely to be involved in rice disease resistance.