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Molecular analysis of arbuscular mycorrhizal fungi colonising a semi‐natural grassland along a fertilisation gradient
Author(s) -
Santos Juan C.,
Finlay Roger D.,
Tehler Anders
Publication year - 2006
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2006.01799.x
Subject(s) - grassland , fertilisation , arbuscular mycorrhizal fungi , biology , arbuscular mycorrhizal , natural (archaeology) , ecology , extremophile , symbiosis , botany , environmental science , microorganism , bacteria , inoculation , paleontology , embryo , reproductive technology , immunology , embryogenesis , microbiology and biotechnology
Summary• The community of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Festuca pratensis and Achillea millefolium was characterized in a Swedish pasture at different times, along a gradient of fertilization. • The small subunit ribosomal RNA gene was subjected to PCR and denaturing gradient gel electrophoresis (DGGE), sequencing and phylogenetic analysis. • The sequences found in this study clustered in 10 discrete sequence groups, seven belonging to Glomus , two to Scutellospora and one to Diversispora . • A negative correlation was observed between soil mineral nitrogen and the number of AMF sequence groups in the roots. The frequency of occurrence of AMF in roots decreased dramatically between June and September. No plant‐host specificity could be detected.