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Peptide uptake in the ectomycorrhizal fungus Hebeloma cylindrosporum : characterization of two di‐ and tripeptide transporters (HcPTR2A and B)
Author(s) -
Benjdia Mariam,
Rikirsch Enno,
Müller Tobias,
Morel Mélanie,
Corratgé Claire,
Zimmermann Sabine,
Chalot Michel,
Frommer Wolf B.,
Wipf Daniel
Publication year - 2006
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2006.01672.x
Subject(s) - biology , ectomycorrhiza , fungus , mycelium , botany , tripeptide , peptide , biochemistry , mycorrhiza , symbiosis , bacteria , genetics
Summary• Constraints on plant growth imposed by low availability of nitrogen are a characteristic feature of ecosystems dominated by ectomycorrhizal plants. Ectomycorrhizal fungi play a key role in the N nutrition of plants, allowing their host plants to access decomposition products of dead plant and animal materials. Ectomycorrhizal plants are thus able to compensate for the low availability of inorganic N in forest ecosystems. • The capacity to take up peptides, as well as the transport mechanisms involved, were analysed in the ectomycorrhizal fungus Hebeloma cylindrosporum . • The present study demonstrated that H. cylindrosporum mycelium was able to take up di‐ and tripeptides and use them as sole N source. Two peptide transporters (HcPTR2A and B) were isolated by yeast functional complementation using an H. cylindrosporum cDNA library, and were shown to mediate dipeptide uptake. • Uptake capacities and expression regulation of both genes were analysed, indicating that HcPTR2A was involved in the high‐efficiency peptide uptake under conditions of limited N availability, whereas HcPTR2B was expressed constitutively.