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Molecular characterization and expression of four cDNAs encoding sucrose synthase from green bamboo Bambusa oldhamii
Author(s) -
Chiu WenBin,
Lin ChiouHong,
Chang ChunJu,
Hsieh MengHsun,
Wang AiYu
Publication year - 2006
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2005.01638.x
Subject(s) - complementary dna , biology , sucrose , gene , biochemistry , microbiology and biotechnology , escherichia coli , cdna library , sucrose synthase , gene expression , invertase
Summary• Bamboo is distinguished by its rapid growth. To investigate sucrose metabolism in this plant, we cloned the cDNAs encoding sucrose synthase (SuS) from Bambusa oldhamii and investigated their expression in growing shoots and leaves. • Four cDNA clones, BoSus1 , BoSus2 , BoSus3 and BoSus4 , were isolated by screening a cDNA library from etiolated bamboo shoots. Recombinant BoSuS proteins were produced in Escherichia coli and purified by immobilized metal affinity chromatography and ultrafiltration. Semi‐quantitative reverse transcriptase–polymerase chain reaction (RT‐PCR) was used to determine the abundance of the transcript of each gene. • BoSus1 and BoSus3 may be duplicate or homeologous genes, the sequences of which show high identity. Similarly, BoSus2 shows high identity with BoSus4 . Kinetic analysis showed that the two BoSuS isoforms of each type had similar michaelis constant ( K m ) values for sucrose, but different values for UDP. The four genes were expressed in various bamboo organs but were differentially regulated. The increase in the abundance of their mRNA paralleled the growth rate of the bamboo. • The results suggest that, in bamboo, SuS is encoded by at least four genes, each with a specific role in providing substrates for the polysaccharide biosynthesis and/or energy production necessary to support the rapid growth of this species.