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A case study from the interaction of strawberry and Botrytis cinerea highlights the benefits of comonitoring both partners at genomic and mRNA level
Author(s) -
Mehli Lisbeth,
Kjellsen Trygve D.,
Dewey Frances M.,
Hietala Ari M.
Publication year - 2005
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2005.01526.x
Subject(s) - botrytis cinerea , fragaria , biology , pathogen , gene , pectinase , botrytis , mycelium , inoculation , microbiology and biotechnology , gene expression , horticulture , botany , genetics , enzyme , biochemistry
Summary• Strawberry Fragaria × ananassa (cv. Korona) was inoculated with Botrytis cinerea by dipping berries in a conidial suspension. • Colonization by the pathogen was monitored using real‐time PCR, ELISA and ergosterol assays, the first showing the highest sensitivity. The expression of pathogen β‐tubulin and six polygalacturonases ( Bcpg 1–6) and three host defence genes (polygalacturonase‐inhibiting protein ( FaPGIP ) and two class II chitinases) were monitored using real‐time RT–PCR. • The maximum transcript levels of the host defence genes occurred at 16 h postinoculation (hpi) at the presumed initial penetration stage. The unique transcript profile of Bcpg 2 over the 96‐h incubation time and its high transcript levels relative to those of the other Bcpg s at 8–24 hpi suggest that the gene has a specific role in the penetration stage. • Bcpg 1 was expressed constitutively at a relatively high level in actively growing mycelia throughout the experimental period. Comparison of the transcript profiles indicated that Bcpg 1 and Bcpg 3–6 were coordinately regulated.