Premium
The transcriptome of Populus in elevated CO 2
Author(s) -
Taylor Gail,
Street Nathaniel R.,
Tricker Penny J.,
Sjödin Andreas,
Graham Laura,
Skogström Oskar,
Calfapietra Carlo,
ScarasciaMugnozza Giuseppe,
Jansson Stefan
Publication year - 2005
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2005.01450.x
Subject(s) - transcriptome , downregulation and upregulation , gene expression , biology , gene , microarray , rubisco , microarray analysis techniques , adaptation (eye) , complementary dna , gene expression profiling , microbiology and biotechnology , botany , genetics , neuroscience
Summary• The consequences of increasing atmospheric carbon dioxide for long‐term adaptation of forest ecosystems remain uncertain, with virtually no studies undertaken at the genetic level. A global analysis using cDNA microarrays was conducted following 6 yr exposure of Populus × euramericana (clone I‐214) to elevated [CO 2 ] in a FACE (free‐air CO 2 enrichment) experiment. • Gene expression was sensitive to elevated [CO 2 ] but the response depended on the developmental age of the leaves, and < 50 transcripts differed significantly between different CO 2 environments. For young leaves most differentially expressed genes were upregulated in elevated [CO 2 ], while in semimature leaves most were downregulated in elevated [CO 2 ]. • For transcripts related only to the small subunit of Rubisco, upregulation in LPI 3 and downregulation in LPI 6 leaves in elevated CO 2 was confirmed by anova . Similar patterns of gene expression for young leaves were also confirmed independently across year 3 and year 6 microarray data, and using real‐time RT–PCR. • This study provides the first clues to the long‐term genetic expression changes that may occur during long‐term plant response to elevated CO 2 .