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The Arabidopsis CDC25 induces a short cell length when overexpressed in fission yeast: evidence for cell cycle function
Author(s) -
Sorrell D. A.,
Chrimes D.,
Dickinson J. R.,
Rogers H. J.,
Francis D.
Publication year - 2005
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01288.x
Subject(s) - arabidopsis , cdc25 , yeast , microbiology and biotechnology , fission , cell cycle , function (biology) , biology , cell , botany , cyclin dependent kinase 1 , genetics , gene , mutant , physics , quantum mechanics , neutron
Summary• The putative mitotic inducer gene , Arath;CDC25 cloned in Arabidopsis thaliana , was screened for cell cycle function by overexpressing it in Schizosaccharomyces pombe (fission yeast). The expression pattern of Arath;CDC25 was also examined in different tissues of A. thaliana . • Fission yeast was transformed with plasmids pREP1 and pREP81 with the Arath;CDC25 gene under the control of the thiamine‐repressible nmt promoter. Using reverse transcription‐polymerase chain reaction (RT‐PCR), the expression of Arath;CDC25 was examined in seedlings, flower buds, mature leaves and stems of A. thaliana ; actin ( ACT2 ) was used as a control. • In three independent transformants of fission yeast, cultured in the absence of thiamine (T), pREP1:: Arath;CDC25 induced a highly significant reduction in mitotic cell length compared with wild type, pREP:: Arath;CDC25 +T, and empty vector (pREP1 ± T). The extent of cell shortening was greater using the stronger pREP1 compared with the weaker pREP81. However, Arath;CDC25 was expressed at low levels in all tissues examined. • The data indicate that Arath;CDC25 can function as a mitotic accelerator in fission yeast. However, unlike other plant cell cycle genes , expression of Arath;CDC25 was not enhanced in rapidly dividing compared with non‐proliferative Arabidopsis tissues.