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Caesium‐affected gene expression in Arabidopsis thaliana
Author(s) -
Sahr Tobias,
Voigt Gabriele,
Paretzke Herwig G.,
Schramel Peter,
Ernst Dieter
Publication year - 2005
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01282.x
Subject(s) - arabidopsis , arabidopsis thaliana , suppression subtractive hybridization , gene , caesium , gene expression , biology , abiotic component , abiotic stress , microbiology and biotechnology , chemistry , biochemistry , botany , cdna library , paleontology , organic chemistry , mutant
Summary• Excessive caesium can be toxic to plants. Here we investigated Cs uptake and caesium‐induced gene expression in Arabidopsis thaliana . • Accumulation was measured in plants grown for 5 wk on agar supplemented with nontoxic and up to toxic levels of Cs. Caesium‐induced gene expression was studied by suppression‐subtractive hybridization (SSH) and RT–PCR. • Caesium accumulated in leaf rosettes dependent upon the external concentration in the growth media, whereas the potassium concentration decreased in rosettes. At a concentration of 850 µ m , Cs plants showed reduced development, and withered with an increase in concentration to 1 m m Cs. SSH resulted in the isolation of 73 clones that were differentially expressed at a Cs concentration of 150 µ m . Most of the genes identified belong to groups of genes encoding proteins in stress defence, detoxification, transport, homeostasis and general metabolism, and proteins controlling transcription and translation. • The present study identified a number of marker genes for Cs in Arabidopsis grown under nontoxic Cs concentrations, indicating that Cs acts as an abiotic stress factor.