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Poplar genes encoding fasciclin‐like arabinogalactan proteins are highly expressed in tension wood
Author(s) -
Lafarguette Florian,
Leplé JeanCharles,
Déjardin Annabelle,
Laurans Françoise,
Costa Guy,
LesageDescauses MarieClaude,
Pilate Gilles
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01175.x
Subject(s) - arabinogalactan , complementary dna , immunocytochemistry , blot , western blot , cdna library , northern blot , xylem , biology , microbiology and biotechnology , gene , cell wall , biochemistry , botany , endocrinology
Summary• Fifteen poplar cDNA encoding fasciclin‐like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of arabinogalactan proteins (AGPs) was globally assessed during wood formation. • PopFLAs transcript accumulation was analysed through EST distribution in cDNA libraries, semi‐quantitative RT‐PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents were globally quantified by rocket electrophoresis. AGPs accumulation was further examined by Western blotting and immunocytolocalization. • Ten PopFLAs were specifically expressed in tension wood (TW) and not expressed in the cambial zone. Rocket electrophoresis revealed important AGPs accumulation in TW xylem. An anti‐AGPs specific antibody recognized two proteins preferentially present in the cell wall‐bound fraction from TW. Immunocytochemistry revealed a strong labelling close to the inner part of the G‐layer of TW fibres. • PopFLAs are expressed in xylem and many are up‐regulated in TW. It is suggested that some PopFLAs accumulating at the inner side of the G‐layer may have a specific function in the building of this layer. PopFLAs expression may therefore be linked to the specific mechanical properties of TW.