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Uptake of nitrogen and carbon from double‐labelled ( 15 N and 13 C) glycine by mycorrhizal pine seedlings
Author(s) -
Taylor A. F. S.,
Gebauer G.,
Read D. J.
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01164.x
Subject(s) - shoot , glycine , nitrogen , carbon fibers , botany , isotopes of nitrogen , isotopes of carbon , mycorrhiza , mycorrhizal fungi , stable isotope ratio , isotope , ectomycorrhiza , biology , symbiosis , chemistry , amino acid , horticulture , environmental chemistry , total organic carbon , biochemistry , bacteria , inoculation , materials science , composite number , composite material , genetics , quantum mechanics , physics , organic chemistry
Summary• While it is accepted that many ectomycorrhizal fungi can assimilate organic substrates and facilitate transfer of their elemental components to plants, the fate of the carbon contained in these materials remains uncertain. Here we investigate the compartmentation of carbon and nitrogen in ectomycorrhizal seedlings of Pinus sylvestris fed with double‐labelled ( 15 N and 13 C) glycine as their sole N source. • Using isotope ratio mass spectrometry, the quantities of N and C derived from this glycine were determined in sequentially harvested samples of mycorrhizas, roots and shoots. • Whereas considerable quantities of 15 N were observed in the mycorrhizal tips, roots and shoots, comparable amounts of 13 C were observed only in mycorrhizal tips and roots. • It is clearly important to resolve the role of compound specificity as a factor determining the extent of amino‐acid C transfer from roots to shoots. However, from the standpoint of the C budget of the whole plant, wherever heterotrophically acquired C is available as an energy source it will reduce demands on photosynthetically fixed sources of the element.