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The impact of ectomycorrhiza formation on monosaccharide transporter gene expression in poplar roots
Author(s) -
Grunze Nina,
Willmann Martin,
Nehls Uwe
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01158.x
Subject(s) - ectomycorrhiza , apoplast , biology , mycorrhiza , transporter , hypha , gene expression , symbiosis , fungus , endodermis , gene , botany , biochemistry , cell wall , genetics , bacteria
Summary• By using degenerate primers, five putative poplar monosaccharide transporter genes were isolated from ectomycorrhizas by RT‐PCR. The expression profiles of the three most strongly expressed ones are presented in detail. • Two transporter genes ( PttMST1.2 and PttMST2.2 ) were down‐regulated by ectomycorrhiza formation. However, PttMST3.1 , which showed 10‐times higher expression rates in noninfected roots than any other transporter gene, was up‐regulated 12‐fold in mycorrhizas. • While changes in PttMST1.2 and PttMST2.2 expression might be regulated by a fungal metabolite present in axenically grown hyphae, the strong increase of PttMST3.1 expression in mycorrhizas required active plant–fungus interaction. • Up‐regulation of PttMST3.1 by mycorrhiza formation suggests that root cells are able to compete with fungal hyphae for hexoses from the common apoplast during symbiosis, redirecting the sugar‐flux back into plant cells whenever the fungal partner does not supply sufficient mineral nutrients. Such a mechanism would enable the plant to link nutrient supply and fungal carbon support at a local level.