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Differential responses of G‐protein Arabidopsis thaliana mutants to ozone
Author(s) -
Booker Fitzgerald L.,
Burkey Kent O.,
Overmyer Kirk,
Jones Alan M.
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01081.x
Subject(s) - heterotrimeric g protein , arabidopsis , arabidopsis thaliana , biology , mutant , protein subunit , microbiology and biotechnology , g protein , gtpase activating protein , botany , biochemistry , signal transduction , gene
Summary• Ground‐level ozone (O 3 ) curtails agricultural production in many regions worldwide. However, the etiology of O 3 toxicity remains unclear. Activated oxygen species appear to inflict biochemical lesions and propagate defense responses that compound plant injury. Because some plant defense responses involve membrane‐delimited GTPases (G proteins), we evaluated the O 3 sensitivity of Arabidopsis mutants altered in the heterotrimeric G‐protein pathway. • Eight genotypes were treated with a range of O 3 concentrations (0, 100, 175 and 250 nmol mol −1 ) for 13 d in controlled environment chambers. • After treatment with O 3 , the epinasty typically observed for wild type leaves did not occur in mutant plants lacking the alpha subunit of the G‐protein complex ( gpa1 ). O 3 ‐induced suppression of leaf chlorophyll levels and leaf mass per unit leaf area were less for gpa1 mutants and were not due to differences in O 3 flux. • There was a positive correlation between the lack of a G‐protein alpha subunit and decreased O 3 sensitivity. Our results suggest that a heterotrimeric G‐protein is critically involved in the expression of O 3 effects in plants.