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24‐Methyl/methylene sterols increase in monoxenic roots after colonization by arbuscular mycorrhizal fungi
Author(s) -
Fontaine J.,
GrandmouginFerjani A.,
Glorian V.,
Durand R.
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01075.x
Subject(s) - sterol , cichorium , daucus carota , glomus , fungus , botany , biology , colonization , phycomycetes , mycorrhiza , symbiosis , bacteria , inoculation , microbiology and biotechnology , cholesterol , biochemistry , horticulture , genetics
Summary• Characteristic sterols of transformed carrot ( Daucus carota ) and chicory ( Cichorium intybus ) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified. • Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC‐MS) from monoxenic cultures of mycorrhizal and nonmycorrhizal roots. After colonization by Glomus intraradices , Glomus proliferum and Glomus sp., carrot and chicory roots exhibited a significantly higher 24‐methyl/methylene sterol content. A correlation was established between the content of the sum of 24‐methyl cholesterol, 24‐methylene cholesterol and 24‐methyl desmosterol. • This study clearly established that the increment of these characteristic sterols is an appropriate indicator of colonization by AM fungi of transformed roots. • Metabolic origin and specificity of these sterols in mycorrhizal roots was researched. The 24‐methyl/methylene sterol increase was observed only when the interaction between fungus and plant was completely established and the fungus was present inside the roots.