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Gene‐specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms
Author(s) -
Hunt L.,
Otterhag L.,
Lee J. C.,
Lasheen T.,
Hunt J.,
Seki M,
Shinozaki K.,
Sommarin M.,
Gilmour D. J.,
Pical C.,
Gray J. E.
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01069.x
Subject(s) - biology , arabidopsis , gene , gene expression , arabidopsis thaliana , gene isoform , gene family , abiotic stress , microbiology and biotechnology , phospholipase c , biochemistry , signal transduction , mutant
Summary• PI‐PLCs synthesise the calcium releasing second messenger IP 3 . We investigated the expression patterns of the Arabidopsis PI‐PLC gene family and measured in vitro activity of encoded enzymes. • Gene specific RT‐PCR and promoter‐GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli . • All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1 , and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1 , 4 and 5 were induced by abiotic stresses. AtPLC1–5 were all shown to have phospholipase C activity in the presence of calcium ions. • AtPLC s showed limited tissue specific expression and expression of at least three genes was increased by abiotic stress. The differing calcium sensitivities of recombinant AtPLC protein activities may provide a mechanism for generating calcium signatures.