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The CBEL elicitor of Phytophthora parasitica var. nicotianae activates defence in Arabidopsis thaliana via three different signalling pathways
Author(s) -
Khatib Moustafa,
Lafitte Claude,
EsquerréTugayé MarieThérèse,
Bottin Arnaud,
Rickauer Martina
Publication year - 2004
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2004.01043.x
Subject(s) - elicitor , jasmonic acid , biology , arabidopsis thaliana , phytophthora nicotianae , oomycete , salicylic acid , arabidopsis , microbiology and biotechnology , plant defense against herbivory , phytophthora , botany , biochemistry , mutant , pathogen , gene
Summary• Phytophthora parasitica var. nicotianae , an oomycete pathogen of tobacco, produces a cell wall glycoprotein named CBEL, which induces necrosis and defence responses in the host plant. Here, we investigated the elicitor activity of CBEL in Arabidopsis thaliana . • CBEL was infiltrated into leaves of Colombia‐0, and its effect on defence responses was assessed. Involvement of the signalling pathways mediated by salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) was estimated by using SA‐deficient transgenic plants ( Nah G) and two mutants affected in the perception of JA ( coi 1) and ET ( ein 2). • We found that CBEL induced necrosis, ethylene biosynthesis, HRGP accumulation, peroxidase activity and expression of the defence‐related genes ACO 1, ASA 1, Wak 1 and PR‐ 1 in Col‐0. Necrosis induction was abolished in coi 1 and ein 2, whereas induction of PR ‐1 and Wak 1 expression, HRGP accumulation and peroxidase activity was affected in Nah G plants. • Our results suggest that the three signalling pathways SA, JA and ET are involved differentially in the induction of necrosis and defence by CBEL in A. thaliana .