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Nitrite assimilation by the yeast Candida nitratophila
Author(s) -
KUBISI ADNAN,
ALI ABID H.,
HIPKIN CHARLES R.
Publication year - 1996
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1996.tb01851.x
Subject(s) - nitrite , nitrate , ammonium , assimilation (phonology) , chemistry , nitrogen assimilation , yeast , carbon source , yeast extract , biochemistry , environmental chemistry , inorganic chemistry , food science , fermentation , organic chemistry , philosophy , linguistics
summary Nitrite uptake in the nitrate‐assimilating yeast, Candida nitratophila , was inhibited completely by addition of nitrate or ammonium to cultures at pH 8.0). Nitrite assimilation in these cultures was also completely dependent on the availability of a suitable carbon source (glucose), In cultures at pH 5.0, neither nitrate nor ammonium inhibited nitrite uptake completely. In these cultures, some nitrite was assimilated in the absence of a carbon source. Carbon‐deficient cultures at pH 5.0 were able to reduce nitrite to ammonium but excreted this ammonium without further assimilation, Evidence for the uptake of nitrite by passive diffusion and by active transport via the nitrate uptake system is discussed.