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Stimulation by auxin and sucrose of bulbil formation in vitro by single leaf cultures of Narcissus
Author(s) -
STAIKIDOU I.,
SELBY C.,
ARVEY B. M. R.
Publication year - 1994
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1994.tb04282.x
Subject(s) - auxin , shoot , sucrose , explant culture , biology , botany , bulb , narcissus , mutant , stimulation , cultivar , horticulture , in vitro , biochemistry , gene , neuroscience
SUMMARY Bulbil formation in vitro by single leaf explains from shoot clump cultures was investigated for Narcissus cultivars St Keverne and Havera. Single leaf explants (30–50 mm leaf with attached basal plate tissue) formed bulbils very infrequently unless cultured on media containing both auxin and a high concentration of sucrose whereas auxin was not necessary for bulbil formation by shoot dump cultures. As well as showing much stronger stimulation by auxin, bulbil initiation and development were more strongly inhibited In BA in single leaf cultures than in shoot dump cultures. Partitioning of dry matter into bulbils was generally lower in single lent cultures than in shoot clump cultures but was more sensitive to growth regulators. Optimal sucrose and auxin concentrations for bulbil development by single leaf cultures were investigated. Bulbil formation in the presence of NAA was strongly stimulated by 176 mM sucrose for both cultivars but there were genotypie differences in auxin responses, St Keverne showing good bulbil development with 0.54 μM NAA, 5.4 μ 1AA and 5.4 μM 1BA und Hawera responding only to 27 μM LAA. It is concluded that single leaf cultures could provide a simple system for investigation of the regulation of the regulation of Narcissus bulb initiation and development.