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Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn.f.
Author(s) -
CHAITANYA K. S. KRISHNA,
NAITHANI SUBHASH CHANDRA
Publication year - 1994
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1994.tb02957.x
Subject(s) - lipid peroxidation , shorea robusta , superoxide dismutase , chemistry , recalcitrant seed , biochemistry , horticulture , botany , food science , biology , oxidative stress , germination
SUMMARY Recalcitrant seeds of Shorea robusta (sal) exhibit 100% viability up to 4 d after maturity. The rapid loss of viability after 4 d is associated with the reduction in moisture content below the lowest safe moisture content (37°o). Seed becomes non‐viable on 8 d. Increased leakage of electrolytes in seeds and lipid peroxidation in embryonic axes was discernible immediately from 0 d. In embryonic axes, very low levels of superoxide (O 2 − ) were maintained up to 4 d and a sharp increase was registered up to 7 d. It is suggested that loss of moisture content in sal seeds below 37% (after 4 d) induces substantial leakage loss probably due to increased lipid peroxidation and O 2 − ) radical formation which are responsible for severe membrane perturbations leading to rapid loss of viability. In embryonic axes SOD activity was recorded only in 100% viable seeds.