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Isolation and purification of intralamellar vesicles from Lemna minor L. chloroplasts
Author(s) -
PICHER M.,
GRENIER G.,
PURCELL M.,
PROTEAU L.,
BEAUMONT G.
Publication year - 1993
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1993.tb03774.x
Subject(s) - galactolipid , vesicle , thylakoid , chloroplast , biology , membrane , stroma , biochemistry , lemna minor , composition (language) , biophysics , botany , phospholipid , chemistry , chromatography , aquatic plant , philosophy , immunohistochemistry , macrophyte , gene , immunology , ecology , linguistics
SUMMARY Intralamellar vesicles (IV) from chloroplasts of Lemna minor L. have been isolated and purified on a sucrose gradient, following sonication. Electron microscopy studies and lipid composition analyses suggest that IV are different in structure and in lipid composition from plastoglobuli. The presence of a high galactolipid composition (70%) suggests that IV are surrounded by a membrane (unit or half‐unit). The ratio of diacylgalactosylglycerol/diacyldigalactosylglycerol (3.9) of IV is similar to that of thylakoid membranes facing the stroma.