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Amino acid and amide metabolism in the hulls and seeds of developing fruits of garden pea, Pisum sativum L.
Author(s) -
Aspartate V.,
MURRAY DAVID R.
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb05662.x
Subject(s) - pisum , sativum , sucrose , threonine , biology , phloem , botany , serine , amino acid , biochemistry , embryo , metabolism , horticulture , enzyme , microbiology and biotechnology
SUMMARY Pea plants (Pisum sativum L. cv. Melbourne Market), each with a single developing pod, were pulse‐fed [4‐ 14 C]aspartate through the cut stem at stages from 11 to 32 d after full blossom. The distribution of radioactivity in soluble metabolites of the hull, seedcoats, embryo sac liquid and embryo was determined by 2‐dimensional paper chromatography and radioautography after feeding intervals of 6 h, 24 h or 48 h. At the earliest stage, radioactivity was recovered mainly in sucrose, serine, homoserine and threonine in the hull. At the 15‐16 d stage, threonine became the major derived amino compound secreted into the embryo sac, and sucrose became the predominant derivative recovered from both hull and seed tissues. Stem‐fed aspartate can therefore be transferred to stem phloem, and sucrose synthesized from aspartate in stems and leaves can be transferred to phloem in both organs.