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Symplastic transport of Lucifer Yellow in mature leaf blades of barley: potential mesophyll‐to‐sieve‐tube transfer
Author(s) -
FARRAR JOHN,
SCHOOT CHRIS van der,
DRENT PETER,
BEL AART
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb05654.x
Subject(s) - sieve tube element , vascular bundle , lucifer yellow , biophysics , sieve (category theory) , botany , fluorescence microscope , chemistry , biology , fluorescence , anatomy , phloem , intracellular , optics , microbiology and biotechnology , gap junction , mathematics , physics , combinatorics
SUMMARY Intermediate veins of mature barley leaves were exposed by partial enzymic removal of mesophyll cells. Lucifer Yellow was iontophoresed into a variety of cell types and its symplastic transfer followed by fluorescence microscopy. Dye transfer from mesophyll cells into the parenchymatous bundle sheath (PBS) and from PBS to mestome sheath (MS) occurred readily. Dye transfer around and along both PBS and MS was rapid. Following injection into PBS or MS dye could be seen moving within sieve tubes, thus providing a demonstration of potential mesophyll‐to‐sieve tube transfer.