Premium
Oriented degradation of the genome of cyanophage AN‐23 after exposure to NaCl
Author(s) -
BANCROFT I.,
SMITH R. J.
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb01127.x
Subject(s) - genome , dna , agarose gel electrophoresis , restriction enzyme , genome size , agarose , restriction fragment , gel electrophoresis , biology , genomic dna , microbiology and biotechnology , restriction map , chemistry , bacteriophage , genetics , plasmid , gene , escherichia coli
summary The low tolerance of cyanophage AN‐23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN‐23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.