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An in vitro assay for incompatibility between Triticum aestivum L. and Secale cereale L.
Author(s) -
OSTASH M.,
FAUTRIER A. G.,
FIELD R. J.
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb00100.x
Subject(s) - secale , germination , pollen , in vitro , pollen tube , biology , botany , poaceae , biochemistry , pollination
summary A liquid medium was developed for in vitro germination and growth of rye (Secale cereale L.) pollen. Addition of style extracts from ‘Otane’ wheat which were incompatible with rye selectively inhibited pollen tube growth, compared with compatible ‘Chinese Spring’ wheat. Dialysed extracts were most suitable for incompatibility assays. Rye pollen was also selectively inhibited by extracts from self–compatible styles. Intergeneric incompatibility in wheat appears to be controlled by inhibitory style proteins.