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Chlorophyll fluorescence decay profiles of O 3 ‐exposed spruce needles as measured by time‐correlated single photon counting
Author(s) -
EVANS E. H.,
BROWN R. G.,
WELLBURN A. R.
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb00079.x
Subject(s) - picea abies , chlorophyll fluorescence , fluorescence , photon counting , ozone , chemistry , chlorophyll , analytical chemistry (journal) , chlorophyll a , wavelength , botany , atmospheric sciences , photon , environmental chemistry , optics , physics , biology , organic chemistry , biochemistry
summary Chlorophyll fluorescence decay profiles have been measured in the wavelength range 680–720 nm for needles from Norway spruce [ Picea abies (L.) Karst] trees which have been exposed to ozone. All profiles required three exponential components of lifetime 100–150 ps, 400–600 ps and 3.5–5.0 ns to fit the experimental data. Compared to control samples, the ozone‐treated needles exhibited a greater amount of the longest‐lived chlorophyll fluorescence and a redistribution in intensity for both the other components from 720 + to 690 nm. These observations are interpreted in terms of disruption of energy transfer and break‐up of light‐harvesting complexes on exposure to ozone. The potential for use of the technique in monitoring forest decline is discussed.