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Activity of ribulose bisphosphate carboxylase/oxygenase from potato cultivars with differential response to ozone stress
Author(s) -
ENYEDI ALEXANDER J.,
ECKARDT NANCY A.,
PELL EVA J.
Publication year - 1992
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1992.tb00078.x
Subject(s) - rubisco , cultivar , oxygenase , ribulose 1,5 bisphosphate , pyruvate carboxylase , carboxylation , ribulose , horticulture , ozone , botany , chemistry , solanaceae , biology , photosynthesis , biochemistry , enzyme , organic chemistry , gene , catalysis
summary The effect of ozone (O 3 ) on the carboxylation activity of ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) from the fifth youngest leaf of four cultivars of Solarium tuberosum L. (potato) was investigated. Ozone‐tolerant cultivars ‘Superior’ (SP) and ‘Norgold Russet’ (NR), and O 3 ‐sensitive cultivars ‘Cherokee’ (CK) and ‘Norland’ (NL) were exposed to O 3 for five consecutive days in controlled‐environment chambers. Leaf samples were obtained during the O 3 ‐exposure period and at 1, 5, and 10 d post‐exposure. For both charcoal‐filtered air (control) and O 3 ‐treated foliage, total activity of Rubisco declined with increasing leaf age. Ozone induced an accelerated decline in the carboxylation activity of Rubisco in leaves obtained from CK and NL by the third day of O 3 exposure. In contrast, O 3 had no impact on the Rubisco activity from leaves of SP and NR as compared to non‐treated foliage. Differential foliar conductance could not account for the response of the sensitive and tolerant cultivars. Rubisco was purified from cultivars SP and CK to determine whether protein sulphydryl content correlated with foliar O 3 sensitivity. The number of SH groups did not differ between the two cultivars, either in chemically denatured or non‐denatured Rubisco. Consequently, neither number nor availability of SH groups appear to be factors controlling sensitivity of Rubisco to oxidative damage by O 3 .