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Morphological aspects of in vitro co‐cultivation of Frankia alni subsp. pommerii Lalonde (strain ACN1 AG ) and Frankia elaeagni (Schröter) Becking (strain EANlpec) with larch ( Larix × eurolepis Henry) cells
Author(s) -
LALIBERTÉ S.,
LALONDE M.
Publication year - 1990
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1990.tb00921.x
Subject(s) - frankia , actinorhizal plant , biology , larch , hypha , strain (injury) , botany , symbiosis , cell wall , microbiology and biotechnology , root nodule , bacteria , genetics , anatomy
summary This study describes the establishment of in vitro co‐cultures between the microsymbiont Frankia and cells from the non‐actinorhizal plant Larix × eurolepis Henry. Co‐cultures with strains from the two host‐plant specificity groups Alnus and Elaeagnus are compared, namely Frankia alni subsp. pommerii Lalonde strain ACN1 AG and Frankia elaeagni (Schroter) Becking strain EANlpec. Larch cell clumps expanded when co‐cultivated with Frankia strain ACNl AG . The actinomvcete proliferated and hyphae developed at the surface of the clumps, in intercellular spaces, in middle lamellae and, more rarely in the plant cell wall. Conversely co‐cultivation of larch cells and Frankia strain EANlpec was highly detrimental for both partners. Dual colonies became degenerate, with the appearance of necrotic plant cells and senescent Frankia structures. The plant cell wall seemed to show a hypersensitive reaction to the presence of the actinomvcete.