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The response to salinity at the microscopic level of the marine fungus Dendryphiella salina Nicot and Pugh as investigated stereologically
Author(s) -
CLIPSON N. J. W.,
JENNINGS D. H.,
SMITH J. L.
Publication year - 1989
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1989.tb02391.x
Subject(s) - hypha , salinity , protoplasm , botany , cytoplasm , biology , volume (thermodynamics) , staining , compartment (ship) , biophysics , cellular compartment , chemistry , microbiology and biotechnology , cell , biochemistry , ecology , physics , oceanography , genetics , quantum mechanics , geology
SUMMARY A stereological analysis of the response of cellular characteristics of hyphae of the marine hyphomycete Dendryphiella salina to salinity is presented. Analyses of volume fractions, compartmental volumes, membrane surface densities and membrane areas have been made. A major finding has been that the wall forms 40 % of the compartment volume. Total hyphal compartment volume was maximal at 200 mM NaCl at 109μm 3 with compartments increasing in width and decreasing in length with salinity. Maximal protoplasmic and cytoplasmic volumes were also found at 200 mM. Electron microscopy demonstrated three different vacuolar types differentiated by their staining characteristics. A light‐staining vacuolar type increased in total volume from 4.5 to 12.2μm 3 from 0 to 1 M NaCl. Data are considered not only in terms of hyphal anatomical characteristics but also in terms of those physiological processes occuring in D. salina which allow it to grow in saline media.