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Early events of vesicular–arbuscular mycorrhiza formation on Ri T‐DNA transformed roots
Author(s) -
BÉCARD G.,
FORTIN J. A.
Publication year - 1988
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1988.tb03698.x
Subject(s) - spore , mycorrhiza , colonization , fungus , biology , arbuscular mycorrhiza , botany , colonisation , phosphorus , arbuscular mycorrhizal , sucrose , mycelium , hypha , symbiosis , horticulture , microbiology and biotechnology , chemistry , bacteria , biochemistry , genetics , organic chemistry
SUMMARY An in vitro system using Ri T‐DXA transformed roots and the vesicular–arbuscular mycorrhizal fungus Gigaspora margarita Becker & Hall has been developed to study the initial events of mycorrhiza formation. Sucrose, sodium and phosphorus were found to be critical components of the medium used to establish the dual culture. Using a single spore as inoculum it was consistently possible to obtain colonization of a preselected point on the root and to time the colonization process (within 5 days). Abundant viable and aseptic spores can be obtained. The system is especially appropriate for studying the triggering of the fungal biotrophy towards the root.