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The isolation of genomic DNA from cyanophage infecting Nostoc and Anabaena species of cyanobacteria
Author(s) -
BANCROFT I.,
SMITH R. J.
Publication year - 1988
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1988.tb00257.x
Subject(s) - cyanobacteria , nostoc , anabaena , biology , genomic dna , dna , isolation (microbiology) , microbiology and biotechnology , restriction enzyme , divalent , genetics , bacteria , chemistry , organic chemistry
SUMMARY The preparation of genomic DNA from a recently isolated group of cyanophage which infect species of Nostoc and Anabaena (cyanobacteria) has been investigated. Factors which affect the yield of DNA, including culture density on infection, divalent cation requirements, NaCl tolerance and desorption from cell debris, have been assessed. These factors have been incorporated into optimized procedures for the isolation of genomic DNA from five cyanophage. The DNA isolated from the rive cyanophage species was identified using restriction endonuclease fragment patterns.