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An in vitro bioassay for the stigmatic product of the self‐incompatibility gene in Papaver rhoeas L.
Author(s) -
FRANKLINTONG V. E.,
LAWRENCE M. J.,
FRANKLIN F. C. H.
Publication year - 1988
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1988.tb00243.x
Subject(s) - bioassay , papaver , biology , pollen , pollen tube , in vivo , in vitro , botany , gene , genetics , pollination
summary This paper reports the development of an in vitro bioassay for the somatic product of the self‐incompatibility gene in Papaver rhoeas L. As with in vivo pollinations, inhibition of pollen tube growth occurs only when the S‐alleles carried by the pollen are matched by those contained in the stigma used to make a print or from which the extract was obtained. Furthermore, these in vitro reactions appear to be quantitatively indistinguishable from those obtained with in vivo pollinations, so that the resolution of the assay allows the distinction between incompatible, half‐compatible and fully compatible reactions to be made on sight rather than by measurement and statistical analysis of pollen tube length.