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PRODUCTION OF FREE STEROLS BY INFECTED TUBERS OF OPHRYSLUTEA CAV.: IDENTIFICATION BY GAS CHROMATOGRAPHY‐MASS SPECTROMETRY
Author(s) -
BARROSO J.,
CHAVES NEVES H.,
PAIS M. SALOMÉ S.
Publication year - 1987
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1987.tb04798.x
Subject(s) - cycloartenol , campesterol , ergosterol , chemistry , chromatography , gas chromatography , stigmasterol , trimethylsilyl , mass spectrometry , gas chromatography–mass spectrometry , sterol , biochemistry , organic chemistry , cholesterol
S ummary Ergosterol, 24–ethyl‐cholest‐7,22‐dien‐3‐β‐ol, campesterol, sitosterol and cycloartenol could be detected in the infected tubers of Ophrys lutea Cav. by capillary gas chromatography and gas chromatography–mass spectrometry of the corresponding O‐trimethylsilyl ethers and acetates. The uninfected tubers only contained demethylsterols. No free sterols could be detected in the endophyte maintained in pure culture. The possible role of sterols in the stability of the host cell membranes in mycorrhizas of O. lutea is discussed.