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HYDROXYLAMINE METABOLISM IN MONORAPHIDIUM BRAUNII
Author(s) -
BALANDIN TERESA,
APARICIO PEDRO J.
Publication year - 1987
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1987.tb02922.x
Subject(s) - hydroxylamine , nitrite , ammonium , anammox , chemistry , photosynthesis , darkness , ammonia , metabolism , biochemistry , nuclear chemistry , botany , organic chemistry , biology , nitrate , nitrogen , denitrification , denitrifying bacteria
S ummary Cells of Monoraphidium braunii L‐egnerova metabolized hydroxylamine, reducing it stoichio‐metrically to ammonium in darkness. In light, the rate of hydroxylamine disappearance was slightly higher than that of the ammonium release to the medium. This may indicate photo‐oxidation of hydroxylamine. Hydroxylamine much impaired ammonium assimilation. Therefore its reduction always resulted in the release of the generated ammonium. Light‐dependent nitrite utilization by the cells was also severely impaired by hydroxylamine, whereas the hydrogen‐dependent nitrite reduction carried out by anaerobic dark‐adapted cells was unaffected. Hence, hydroxylamine does not appear to inhibit nitrite reductase. There was a close correlation between the kinetics of nitrite utilization and those of oxygen evolution. Hydroxylamine prevented photosynthetic cell growth, but when the cells had removed hydroxylamine from the medium growth resumed.