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IMPROVED HYPHAL GROWTH OF TWO SPECIES OF VESICULAR‐ARBUSCULAR MYCORRHIZAL FUNGI IN THE PRESENCE OF SUSPENSION‐CULTURED PLANT CELLS
Author(s) -
CARR G. R.,
HINKLEY M. A.,
TACON F.,
HEPPER C. M.,
JONES M. G. K.,
THOMAS E.
Publication year - 1985
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1985.tb02848.x
Subject(s) - biology , glomus , hypha , chlamydospore , phycomycetes , botany , mycorrhiza , symbiosis , fungus , spore , horticulture , bacteria , inoculation , genetics
S ummary Conditions required for the combined culture of either Glomus caledonium (Nicol. &Gerd.) Trappe &Gerdemann or Glomus mosseae (Nicol. &Gerd.) Gerdemann &Trappe with suspension‐cultured plant cells have been investigated. Sucrose levels (0.05 to 0.5%, w/v) lower than those used for growth of plant cells were optimal for hyphal growth of both G. caledonium and G. mosseae. In vitro hyphal growth from chlamydospores of G. caledonium was stimulated by addition of cells of wheat ( Triticum aestivum L. cv. Maris Butler), lucerne ( Medicago sativa L. cv. Europ) and potato ( Solanum tuberosum L. cv. Maris Piper). The presence of wheat cells similarly stimulated hyphal growth from chlamydospores of G. mosseae. Further tests on the effect of lucerne cells on G. caledonium indicated that a volatile substance was involved in the improvement of hyphal growth.