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EFFECT OF IRRADIANCE AND LIGHT QUALITY ON STARCH SYNTHESIS BY ISOLATED CHLOROPLASTS OF CODIUM FRAGILE
Author(s) -
WILLIAMS MICHAEL L.,
COBB A. H.
Publication year - 1985
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1985.tb02817.x
Subject(s) - starch , photosynthesis , chloroplast , amylase , carotenoid , dehydrogenase , chemistry , botany , biochemistry , biology , enzyme , gene
S ummary Net starch synthesis by isolated chloroplasts of the intertidal alga Codium fragile (Suringar) Hariot was determined by enzymic hydrolysis (amyloglucosidase/α‐amylase) of solubilized chloroplast samples followed by the measurement of the resultant glucose yields by hexokinase/glucose‐6‐phosphate dehydrogenase‐coupled reactions. Net starch synthesis was only observed between 100 and 375 μmol m −2 s −1 (PAR) corresponding to irradiance values which supported saturating or super‐saturating rates of photosynthesis. Light filter inactivation of the light‐harvesting complex carotenoids siphonoxanthin and siphonein reduced net starch synthesis by 68 %. These findings are discussed in relation to photosynthesis in an intertidal zone environment.